Crystallization and Structure Determination of the Human Estrogen Receptor by X-ray Diffraction.
Abstract
This project is a postdoctoral training fellowship for Dr. Robert Nolte, to support X-ray crystallographic studios of cancer-related macromolecular complexes. We have prepared crystals of a complex between a six Zn-finger segment of the transcription factor TPIIIA and a 31 BP DNA site. The determination of this structure is almost complete at an intermediate resolution, with the position of the DNA and protein elements determined. A novel interaction between one of the zinc fingers and the minor groove has been observed. The higher resolution details of the structure, which will be determined in the next several months, should reveal significant now aspects of DNA recognition by Zn-finger proteins. These interactions are responsible for the regulation of many loci important for cell growth. During the final project year of this training grant, Dr. Nolte to will conclude the TFIIIA studies and focus his attention on structural studios on another protein ZAP-70, a T-cell tyrosine kinase which plays a crucial role in signal transduction.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1996
- Accession Number
- ADA320651
Entities
People
- Stephen C. Harrison