The Role of p53 in Human Breast Cancer.
Abstract
The principal objectives are to: (1) Transfect p53 mutants commonly observed in human breast cancer into normal human mammary epithelial cells obtained from different donors and isolate clones; (2) Characterize the clones for extension of lifespan and immortalization; (3) Determine in breast epithelial cells immortalized and expressing mutant pS3 if expression of the mutant p53 is necessary for the maintenance of growth; (4) Determine if expression of any of the p53 mutants provide a selective growth advantage to breast epithelial cells prior to immortalization; (5) Determine downstream genomic targets of p53 that may be important in the development and progression of breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1996
- Accession Number
- ADA321164
Entities
People
- Jerry W. Shay
Organizations
- University of Texas Southwestern Medical Center