Effect of Metals on the Biotransformation of Chlorinated and Non-chlorinated Aromatic Compounds by Anaerobic Bacterial Consortia.
Abstract
Heavy METAL Pollution often co-occurs with aromatic pollution. Therefore, we investigated the effect of added Cd(II), Cr(VI), Cu(II) and Hg(II) on biodegradation of 3-chlorobenzoate (3CB), 2-chiorophenol (2CP), phenol, and benzoate. Cr(VI) was abiotically reduced to Cr(III) in our anaerobic medium, and Cu(II) partially reduced to Cu(I). Complete anaerobic biodegradation of 3CB and 2CP is performed by an interdependent cooperation of anaerobic bacterial strains via 3CB benzoate acetate/H2/002 methane; and 20P phenol benzoate acetate/H2/C02 methane. Target compounds were not degraded at >5 - 100ppm, while less than O.1ppm Cd(II), Cu(II), or Cr(III) had no effect. O.lppm Hg(II) inhibited 2CP and phenol, but not 3CB or benzoate, biodegradation. Cd(II) and Cr(III) inhibited 3CB dechlorination most strongly. Cu(II) and Hg(II), respectively, inhibited benzoate and phenol biodegradation most strongly. Utilization of acetate was most sensitive to Cu(II) and Hg(II) . Methane production was most sensitive to Hg(II). No correlation existed between the initial Eh created by adding metal salts to anaerobic medium and observed inhibition of target compound biodegradation. 2cP and 3CB biodegradation became resistant to Hg(II) by an inducible, transferable, biological activity which is under further investigation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 1996
- Accession Number
- ADA321222
Entities
People
- Barbara R. Genthner
Organizations
- University of West Florida