Effect of HSP27 on Human Breast Tumor Cell Growth and Motility.
Abstract
This award is a Predoctoral Fellowship to support the doctoral training of Donna Egender. The goal of this research is to investigate the effects of the small stress protein, HSP27, on growth and motility characteristics of normal and tumor-derived human mammary cell lines. Our study is based on the hypothesis that HSP27 is a component of a signal transduction pathway that regulates actin microfilament dynamics. We hypothesize that cells overexpressing HSP27 will show increased motility and altered chemotactic properties, in addition to increased resistance to heat killing and certain drugs. Research completed this year includes optimization of transfection of human MCF7 and MDA231 cells, characterization of sensitivity of mammary cell lines to reagents used in selecting stable transfected cell lines, and selection and assay of clonal cell lines. Donna is introducing the use of adenovirus-mediated transfection, for improved efficiency of transfer of genes into breast cell lines. She has also initiated assays of HSP27 promoter function.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1996
- Accession Number
- ADA321344
Entities
People
- Eileen Hickey
Organizations
- University of Nevada, Reno