Protein Kinase C Processes and Their Relation to Apoptosis in Human Breast Carcinoma Cells.

Abstract

It was reported previously that the specific aim of screening agents that would induce apoptosis in the human breast carcinoma cell line MDA-MB-231 was completed, and that calyculin A was the most effective compound tested. Since then Cdc25 phosphatases were reported as possible key oncogenes in human breast carcinoma. In light of our results with calyculin A, it was decided that a more promising focus for the project would be the biochemical basis for the oncogenic actions of Cdc25 phosphatases in human breast carcinoma. At this time we have examined 18 compounds and discovered a Cdc25 phosphatase inhibitor, called 1f, that selectively inhibits Cdc25A,B, and C, has antiproliferative activity against and causes a G1 block in MDA-MB-231 cells. The pure form of this compound and two other compounds which selectively inhibit Cdc25 phosphatases have been made and also demonstrate an equal if not better inhibition of Cdc25 phosphatases as their combinatorial form.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1996
Accession Number
ADA321394

Entities

People

  • John S. Lazo
  • Robert L. Rice

Organizations

  • University of Pittsburgh

Tags

DTIC Thesaurus Topics

  • Alkenes
  • Amines
  • Amino Acids
  • Apoptosis
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Health Services
  • Neoplasms
  • Organic Chemistry
  • Proteins
  • Small Molecules

Fields of Study

  • Biology

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Computer Science.
  • Molecular Biology and Genetics