Genetic Immunization for Lentiviral Immunodeficiency Virus Infection and Disease.

Abstract

In year two of this project we have developed and characterized four novel SIV envelope expression vectors and one vector expressing both envelope and core components for genetic immunization of rhesus monkeys. Experiments using test antigens to determine optimum DNA vaccine parameters are near completion. Beginning in January 1997 we will test one or more of these SIV DNA vaccines for protection against SIV challenge infection via systemic (IV) and mucosal (vaginal) routes of administration. As a potential secondary challenge, should the vaccinated monkeys be protected against homologous SIV, we have developed a pathogenic SIV/HIV (SHIV) envelope recombinant. Cytokine profiles of acutely infected monkeys have been characterized and a large number of rhesus cytokine, B-chemokine and chemokine receptor genes have been cloned, sequenced and recombinant molecules prepared for possible use as immune modulators or adjuvants.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1996
Accession Number
ADA321591

Entities

People

  • Murray B. Gardner

Organizations

  • University of California

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DTIC Thesaurus Topics

  • Biomedical And Dental Materials
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Health Services
  • Lymphatic System
  • Lymphocytes
  • Polymer Chemistry
  • Polymeric Films
  • Vaccines
  • Viruses

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  • Biology
  • Medicine

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  • Immunology
  • Molecular Genetics

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  • Biotechnology
  • Biotechnology - Cancer Biotech