Effects of Hydrostatic Pressure on Mammalian Tissue Cells-Disruption of Cytoskeletal Function, Organization, and Regulation.

Abstract

To investigate the disruption, organization and regulation of the cytoskeleton of mammalian tissue cells by hydrostatic pressure. APPROACH: Novel optical and fixation chambers are being used in combination with immunofluorescence and video microscopy, biochemical and biophysical techniques to examine pressure-induced changes in the structural organization of the cytoskeletal proteins (including tubulin, actin, myosin II, vinculin, talin, vimentin, and cytokeratin) involved in producing changes in cell shape, motility and contractility.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1996
Accession Number
ADA321595

Entities

People

  • Albert K. Harris
  • Edward D. Salmon

Organizations

  • University of North Carolina at Chapel Hill

Tags

DTIC Thesaurus Topics

  • Adhesion
  • Assembly
  • Cell Movement
  • Cell Shape
  • Cells
  • Cytoskeletal Proteins
  • Cytoskeleton
  • Filaments
  • Fluorescent Dyes
  • High Resolution
  • Hydrostatic Pressure
  • Leading Edges
  • Microscopy
  • North Carolina
  • Proteins
  • Regulations
  • Tissues

Fields of Study

  • Biology

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Materials Science and Engineering.
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology