A Genetic Approach to Identifying Signal Transduction Mechanisms Initiated by Receptors for TGF-B-Related Factors.

Abstract

A genetic screen for new mutations affecting the signaling pathway by which cells respond to TGF-beta-related factors in Drosophila was carried out. The genetic background was sensitized using a mutation in the Type I receptor gene. New mutations affecting the phenotypes caused by the sensitized genetic backgrounds were recovered. The new mutations were mapped to six different genes. Three of these genes were previously shown to participate in the signaling pathway, confirming that the sensitized genetic background was appropriate. DNA sequencing is being used to confirm the generation of new mutant alleles in the Mad gene and the Type II receptor gene. Three of the genes in which new mutations were recovered map to regions not previously known to be involved in dpp signaling. Detailed genetic mapping has provided cloning strategies for the molecular identification of two of these genes.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1996
Accession Number
ADA321906

Entities

People

  • F. M. Hoffman

Organizations

  • University of Wisconsin–Madison

Tags

DTIC Thesaurus Topics

  • Animals
  • Biomedical Research
  • Cell Physiological Processes
  • Cells
  • Chromosomes
  • Drosophila
  • Genes
  • Genetic Mapping
  • Genetic Phenomena
  • Genetic Structures
  • Genetics
  • Growth Factors
  • Materials
  • Neoplasms
  • Peptide Growth Factors
  • Peptides
  • Phenotypes

Fields of Study

  • Biology

Readers

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  • Molecular and genetic basis of cancer.

Technology Areas

  • Biotechnology