Breast Cancer Cell Metabolism Studies by MRS.
Abstract
It is proposed to study the metabolism of intact breast cancer cells using non-invasive magnetic resonance spectroscopy (MRS) methods. Magnetic resonance imaging (MRI) has been shown to have some value in assessing breast lumps, but lacks specificity. MRS can in principle provide this specificity. In order to evaluate the potential of MRS, systematic studies will be carried out on cell lines selected for the progressive estrogen independent phenotype and the effects of hormones and tamoxifen. The cell studies will provide basic information on the metabolic profile of different BC cell lines in the progression from estrogen and drug sensitive to estrogen independent and drug resistant phenotypes. Cells are embedded in gels and are examined in the MR spectrometer while undergoing perfusion. Based on preliminary results, we will use a protein gel (Matrigel) for which the cells have surface receptors, so that their metabolism can be monitored while they are proliferating. Both 31P and proton MR methods will be applied.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1996
- Accession Number
- ADA322245
Entities
People
- Jack S. Cohen
Organizations
- Georgetown University