A Medical Research and Evaluation Facility and Studies Supporting the Medical Chemical Defense Program. A Comparison of the Biochemical Changes Produced by Vesicating and Non-Vesicating Alkylating Agents in Normal Human Epidermal Keratinocytes.

Abstract

Task 91-23 was conducted for the U.S. Army Medical Research Institute of Chemical Defense (USAMRICD) to compare alterations in selected biochemical endpoints using human epidermal keratinocytes (HEK) exposed in vitro to either non-vesicating (e.g., cisplatin) or vesicating (e.g., sulfur mustard; MD) alkylating agents. These data may provide insight into the biochemical mechanism(s) uniquely associated with tissue vesication. Preliminary in vivo experiments used three vesicating and three non-vesicating alkylating agents to confirm vesication potential by examining microblister formation in the hairless guinea pig (HGP). The three vesicating, alkylating agents selected were MD (positive control), mechlorethamine (nitrogen mustard; HN2), and chloroethylethyl sulfide (CEES). The three non-vesicating, alkylating agents selected were cisplatin, meiphalan, and thiotepa

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1997
Accession Number
ADA322299

Entities

People

  • Carl T. Olson
  • Frances M. Reid
  • James A. Blank
  • John B. Johnson
  • Ronald G. Menton

Organizations

  • Battelle Memorial Institute

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Alkanes
  • Alkylating Agents
  • Animals
  • Biomedical Research
  • Cells
  • Chemical Analysis
  • Chemical Synthesis
  • Chemistry
  • Deoxyribonucleic Acids
  • Epidermis
  • Experimental Design
  • Liquid Chromatography
  • Medical Personnel
  • Nitrogen Mustards
  • Research Facilities
  • Rodents

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