Genetic Evidence of Early Breast Cancer.
Abstract
A precise understanding of the genetic changes which herald incipient transformation would be invaluable for accurate early diagnosis and a better understanding of breast cancer pathogenesis. Towards this goal, we tried to identify clonal breast populations based on the detection of only one X- chromosome androgen receptor allele after differential methylation sensitive restriction enzyme digestion. Although this analysis was suitable for large amounts of DNA, it was unreliable when small cell numbers were analyzed. A novel strategy to dilute the DNA molecules to essentially single alleles after restriction digest prior to PCR was able to identify small clonal and polyclonal populations but was extremely time consuming. Further development of this strategy may improve the ability to reliably identify small clonal cell populations in the breast.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1996
- Accession Number
- ADA322783
Entities
People
- Darryl Shibata
Organizations
- University of Southern California