Studies on Bacterial Spore Resistance and Regulation of Activation.
Abstract
Highlights of work on spores of Bacillus subtilis in the past performance period have included demonstration that: (1) the zymogen of the spore protease GPR autoactivates, and that autoactivation is stimulated by low pH, dipicolinic acid and dehydration; (2) most of the propeptide of GPR is not needed for zymogen stability or autoprocessing; (3) the decrease in forespore pH in B. subtilis sporulation is responsible for 3-phosphoglycerate accumulation; (4) alpha/ beta-type small, acid-soluble proteins in spores protect DNA against killing by freeze-drying, dry heat and several organic hydroperoxides, by preventing DNA damage; (5) DNA repair during spore germination is an important component of resistance of spores to agents that kill spores through DNA damage; and (6) spore resistance to peroxides is not due to the presence of protective enzymes such as catalases or alkylhydroperoxide reductases in spores.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1997
- Accession Number
- ADA322933
Entities
People
- Peter Setlow
Organizations
- University of Connecticut Health Center