Structural Analysis of the Human T-Cell Receptor/HLA-A2/Peptide Complex.

Abstract

During the second year of this grant, we obtained better X-ray data from the crystals of the T-cell receptor/HLA-A2/peptide complex, by finding improved conditions for freezing the crystals, by producing heavy atom-containing crystals, and by collecting data at the Cornell High Energy Synchrotron Source (CHESS). By the combination of the techniques of molecular replacement, multiple isomorphous replacement, and iterative real-space averaging, we were able to determine the structure of the TCR/HLA-A2/peptide complex. We are in the process of analyzing the immunological implications of the structure and are determining two closely related structures to allow a fuller interpretation of this central molecular recognition event in the immune system.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1996
Accession Number
ADA323769

Entities

People

  • David N. Garboczi
  • Don C. Wiley

Organizations

  • Harvard College

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antigens
  • Cells
  • Charge Coupled Devices
  • Crystals
  • Detectors
  • Electron Density
  • High Energy
  • Immune System
  • Leukocytes
  • Lymphocytes
  • Molecules
  • Peptides
  • Polyethylene Glycols
  • Recognition
  • Synchrotrons
  • T Lymphocytes
  • X Rays

Fields of Study

  • Chemistry

Readers

  • Distributed Systems and Data Platform Development
  • Quantum Chemistry
  • Systems Analysis and Design

Technology Areas

  • Space