Cutaneous Uptake of 14C-HD Vapor by the Hairless Guinea Pig.
Abstract
The hairless guinea pig (HGP) is used by our laboratory to model the human cutaneous response to sulfur mustard (HD) exposure. We have determined the HD content in the skin of HOP after 7-minute exposures to vapors saturated with a mixture of HD and 14C-HD. Concentration/time (C1) values in the range of 2 mg/sq cm/min were determined by counting skin 14C disintegrations per minute (dpm) in animals euthanized immediately after exposure. These values are similar to human penetration rates obtained by other investigators. A direct relationship between C1 and relative humidity was demonstrated in 5 of 6 studies. A rate curve monitoring the reduction in skin 14C dpm was developed for animals euthanized between 0 and 24 hours post exposure. This curve showed the greatest change after 1 hour. Epidermal and dermal distribution of 14C at 24 hours was measured for two animals. Site preference for HD penetration, multiple use of a vapor cap containing HD, and 14C content of adhesive tape were also investigated with radiolabeled HD to evaluate other aspects of the experimental model. These results contribute to a better understanding of the cutaneous response to HD in the HGP model.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1996
- Accession Number
- ADA323919
Entities
People
- Charles B. Millard
- Michael B. Shutz
- Rodolfo Bongiovanni
- Susan M. Schultz
- Thomas P. Logan
Organizations
- United States Army Medical Research Institute of Chemical Defense