Analysis of Multistep Mammary Tumorigenesis in Wnt-1 Transgenic Mice.

Abstract

Mouse mammary tumor virus is used as a mutagen in transgenic mice that carry an activated Wnt-1 gene, to produce additional events such as activation of a second oncogene by nearby insertion of proviral DNA. Cloning cellular sequences flanking a new proviral insertion from tumor 76, identified a common insertion locus for MMTV. Further analyses of this region identified the proto-oncogene Fgf3 to be activated over a long range by the MMTV insertions. Fgf8 is another proto-oncogene that we identified to be activated by MMTV in 10% of these tumors. Characterization of the oncogenic potentials of Fgf8 isoforms in mammary epithelial cells showed a surprising finding; the mammary epithelial cells stimulated by Fgf8b protein containing conditioned medium went through programmed cell death or apoptosis.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1996
Accession Number
ADA323959

Entities

People

  • Deepa Shankar

Organizations

  • Children's Hospital Los Angeles

Tags

DTIC Thesaurus Topics

  • Apoptosis
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Techniques
  • Epithelial Cells
  • Genetically Modified Organisms
  • Genetics
  • Health Services
  • Mammary Glands
  • Neoplasms
  • Polymerase Chain Reaction
  • Programmed Cell Death
  • Proteins
  • Sequences

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics