Destruction of Spoilage and Pathogenic Bacteria by Hydrostatic Pressure and Electroporation in Combination with Biopreservatives. Phase 2.

Abstract

Escherichia coli O157:H7, Salmonella typhimurium ATCC 14028, Listeria monocytogenes Scott A, Leuconostoc mesenteroides Ly and Lactobacillus sake FMl were evaluated for their sensitivity to either hydrostatic pressure (HP) or electroporation (EP) in combination with temperature, biopreservative and lysozyme. Both HP and EP caused viability loss and sublethal injury to bacterial cells. A combination of HP and mild temperature induced greater sublethal injury to cells. Sublethally injured cells became sensitive to a biopreservative and lysozyme. By combining biopreservative and lysozyme, EP at 15 kV/cm for 30 microsecond caused viability loss of 3.0 logs in Salmonella typhimurium and 2.2 logs in Escherichia coli. The viability loss reached 4.3 logs for Escherichia coli and 10.9 logs for Listeria monocytoqenes when cells were exposed to 30,000 lb/in squared at 35 deg C in the presence of biopreservative and lysozyme.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1997
Accession Number
ADA324258

Entities

People

  • Anthony Sikes
  • Bibek Ray
  • C. P. Dunne
  • Norasak Kalchayanand

Organizations

  • University of Wyoming

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Bacteria
  • Bacterial Infections
  • Bacteriology
  • Escherichia
  • Escherichia Coli
  • Gram-Negative Bacteria
  • Gram-Positive Bacteria
  • Heat Treatment
  • Hydrostatic Pressure
  • Listeria Monocytogenes
  • Listeriosis
  • Microbiology
  • Muramidase
  • Pathogenic Bacteria
  • Viability

Readers

  • Microbial Pathology
  • Plasma Physics.
  • Tribology (the study of the boundary interaction between sliding surfaces, lubrication, wear and friction).