Genetic Regulation in the Aiptasia pallida Symbiosis - Performance Report, Year 1.

Abstract

This report describes the progress of the project 'Genetic Regulation in A. pallida Symbiosis'. The main goal of the project in year 1 was to identify sequence tags for differentially expressed genes using the SAGE approach. The initial protocal we followed to compare gene expression in cultured and symbiotic zooxanthellae is one developed for serial analysis of gene expression (SAGE). We initially tested the SAGE protocol with cDNA generated from K. rotundatum. Unfortunately, to date little progress has been made towards meeting our initial goal. The application of the SAGE technique proved technically difficult. We are now focusing on constructing representative cDNA libraries from cultured and symbiotic zooxanthellae and will sequence from both the 3' - and 5'-end approximately 5,000 clones from each library and compare the results. This work will be conducted throughout years 2 and 3.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1997
Accession Number
ADA325661

Entities

People

  • Jean-francois Tomb

Organizations

  • J. Craig Venter Institute

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Classification
  • Gene Expression
  • Genetic Structures
  • Military Research
  • Monitoring
  • Regulations
  • Security
  • Sequences

Readers

  • Microbial Pathology
  • Military History of the United States in the 20th Century.
  • Molecular and genetic basis of cancer.

Technology Areas

  • Biotechnology