Molecular Genetics of Breast Neoplasia.
Abstract
The overall objective of this grant is to develop new multiparametric fluorescence in situ hybridization (M-FISH) techniques for the analysis of chromosomal abnormalities and genetic alterations in breast carcinoma cells and tissues. During year 02, we continued to refine and validate the M-FISH procedure for karyotyping metaphase chromosomes in which each chromosome is identified by a unique spectral signature that is generated by simultaneous hybridization of 24 differentially labeled chromosome specific 'painting' probes. Both simple and highly complex chromosomal aberrations can be detected readily by M-FISH. Extension of M-FISH to fresh or paraffin-embedded tissues is in progress and is largely focused on the development of novel software that combines z-axis optical sectioning, image deconvolution, 3-D image reconstruction and spectra signature extraction. We have also completed an analysis of 27 primary breast carcinoma samples for abnormalities in mRNA transcribed from the FHIT (fragile histidine triad) gene, a candidate tumor suppressor gene mapped to the 3p14 region of the genome. Aberrant FHIT RNAs were detected in 40% of the tumors examined, suggesting that the FHIT gene may be involved in the progression of a subset of breast carcinomas.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1996
- Accession Number
- ADA325931
Entities
People
- David C. Ward
Organizations
- Yale University