Electrical and Chemical Modulation of Synaptic Efficacy.
Abstract
To investigate how electric currents associated with Synaptic activity and chemical factors released by the pre- and postsynapic cells affect the efficacy of synaptic transmission, in order to understand the plasticity of synaptic functions at single neuron level. Monolayer cultures of dissociated neurons from rat hippocampi or of neurons and myocytes from Xenopus embryos are prepared, the synaptic functions are assayed by patch-clamp whole-cell recording and fluorescence imaging of intracellular calcium. Electrical activity is induced in the neuronal network by extracellular or intracellular stimuli and chemical factors are bath- or focally-applied to the synapses.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 13, 1997
- Accession Number
- ADA326974
Entities
People
- Mu-ming Poo
Organizations
- Columbia University