Construction and Analysis of Recombinant Mouse Anti-Botulinum Antibodies.

Abstract

Anti-botulinum neurotoxin antibodies were produced by cloning heavy and light chain genes from an immunized mouse. Selection by phage display against immobilized botulinum toxin subtype B allowed for the isolation of multiple high affinity Fab producing cell lines. These clones can be directed to express large quantities of Fab antibody at a significantly lower cost than hybridoma cell culture. Purification can be simplified by engineering an affinity tag directly into the nucleotide sequence of each antibody clone. Methods of construction and analysis are presented along with affinity constant determination and nucleotide sequencing data.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1997
Accession Number
ADA327459

Entities

People

  • Darrel E. Menking
  • James J. Valdes
  • Jennifer G. Wilson
  • Jessica Dang
  • Peter A. Emanuel

Organizations

  • Edgewood Chemical Biological Center

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical And Dental Materials
  • Cell Line
  • Cells
  • Chemical Compounds
  • Chemical Synthesis
  • Chemistry
  • Construction
  • Detection
  • Engineering
  • Genetic Engineering
  • Genetic Structures
  • Immunoglobulins
  • Molecular Biology
  • Proteins
  • Surface Plasmon Resonance
  • Surface Plasmons

Fields of Study

  • Biology

Readers

  • Immunology
  • Microbial Pathology
  • Molecular Genetics