Comparative Roles of Overexpressed and Mutated H- and K-ras in Mammary Carcinogenesis.

Abstract

The objective of this study was to characterize events in the uniformly selective association of H-ras gene activation, but not K-ras gene activation, in the rat mammary carcinogenesis model. Three primary objectives were investigated. First, the relative tumorigenic potency of activated H-ras and K-ras-2B was investigated. The activated H-ras gene was found to be approximately 10-fold more potent at inducing mammary tumors than the activated K-ras gene. Yet, the K-ras oncogene was still effective at mammary carcinoma induction. Second, the relative percentage of mammary transplants with mutations in H-ras and K-ras was investigated. This objective will be completed soon, but, as yet, no data is available. The third objective was to investigate the role of H-ras regulatory elements in rat mammary tumorigenesis using transgenic rats harboring a H-ras gene (HrHr transgenics) or K-ras gene (HrKr transgenics) controlled by H-ras gene regulatory elements.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1996
Accession Number
ADA327718

Entities

People

  • Michael N. Gould
  • Todd A. Thompson

Organizations

  • University of Wisconsin–Madison

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Amino Acids
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Carcinogens
  • Cells
  • Chemical Reactions
  • Gene Expression
  • Genetically Modified Organisms
  • Mammary Glands
  • Materials
  • Medical Personnel
  • Neoplasms
  • Polymerase Chain Reaction
  • Small Molecules
  • Tissues

Readers

  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology