Predictive Binding Parameters for DNA-DNA Association within a Fluid Stream.

Abstract

The ability to predict the rates of association of DNA to DNA have been used previously for those reactions occurring in a test tube. This study shows the ability to predict the binding rate of DNA to DNA within a fluid stream. The primary ligand was an oligonucleotide 20 basepairs attached to the dextran matrix in BlAcore. Efficiency of hybridization of this ligand to a secondary ligand of 40 basepairs (containing 20 complementary) was assessed. Association was predictable, based on ssDNA remaining at equilibrium, using second order rate kinetics. Changes in concentration encompassing one order of magnitude had little to no effect on the efficiency of hybridization.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1997
Accession Number
ADA328050

Entities

People

  • Sheila J. Wood

Organizations

  • Edgewood Chemical Biological Center

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Chain Reactions
  • Chemical Reactions
  • Classification
  • Conversion
  • Detection
  • Detectors
  • Efficiency
  • Environmental Protection
  • Flow Rate
  • Hybridization
  • Instructions
  • Kinetics
  • Materials
  • Nucleic Acids
  • Polymerase Chain Reaction
  • Technical Information Centers

Fields of Study

  • Biology

Readers

  • Computational Modeling and Simulation
  • Fluid Dynamics.
  • Molecular Genetics