Characterization of Light and Nitrogen Regulated Gene Expression Pathways in Marine Diatoms.
Abstract
This report summarizes the results of ONR funded research focused on characterization of the genes and gene products for several biosynthetic and structural proteins found in marine diatoms with the goal to utilize molecular probes generated from these studies to monitor molecular events regulating the production dynamics of marine phytoplankton. The diatom Skeletonema Costatum, an ubiquitous coastal species was selected as a model system. The polymerase chain reaction utilizing consensus oligonucleotide primers to conserved amino acid domains was employed and used to isolate genomic and cDNA fragments corresponding to the sequences encoding the nitrogen assimilatory enzymes nitrate reductase (NR) and glutamine synthetase (GS) and the structural proteins actin and the fucoxanthin chlorophyll a/c protein (FCP).
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1992
- Accession Number
- ADA328090
Entities
People
- G. J. Smith
Organizations
- University of Chicago