A Medical Research and Evaluation Facility and Studies Supporting the Medical Chemical Defense Program. Evaluation of the Utility of Human Skin Equivalents for Studying HD-Induced Dermatotoxicity and Evaluating Antivesicant Treatment Regimens.
Abstract
Two human skin culture models, also known as human skin equivalents (HSEs), and dermatomed (split thickness) natural human skin (NHS) were assessed for use in studies investigating the dermatotoxic effects of sulfur mustard BIS(2-CHLOROETHYL) SULFIDE; HD. The HSEs examined were model ZK1300 from Advanced Tissue Sciences (ATS, La Jolla, CA) and Epiderm from MatTek Corporation (Ashland, MA). NHS specimens were obtained either fresh from the Ohio State University branch of the Cooperative Human Tissue Network (Columbus, OH), or cryopreserved from the Ohio Valley Tissue and Skin Center (OVTSC, Cincinnati, OH). The utility of each skin model was assessed by histopathology and by several viability indices. Tissue samples were processed for both light and electron microscopy and examined for consistency among HSE lots and among NHS patients in exhibiting normal ultrastructural details. In particular, the samples were examined for the presence of a continuous, intact basement membrane and an epidermal-dermal interface resembling that found in normal, living human skin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA328564
Entities
People
- Carl T. Olson
- Christopher A. Logel
- David W. Hobson
- John B. Johnson
- Thomas H. Snider
Organizations
- Battelle Memorial Institute