Modulation and Expression of Tumor Suppressor Genes by Environmental Agents.
Abstract
Studies were undertaken to evaluate the potential role of the retinoblastoma tumor suppressor gene/protein during chemical carcinogenesis in the medaka (Oryzias latipes), a small fish. Cell lines from the rainbow trout liver and long-term primary cultures of medaka liver cells were established to aid these studies. To better understand the role of the retinoblastoma protein during the cell cycle it was necessary to purify the protein from trout liver, develop antibodies, and evaluate protein expression following treatment of fish liver cells with known promoters. Finally, appropriate molecular biological diagnostic reagents were developed to evaluate alterations in the retinoblastoma gene in retinoblastoma and hepatocarcinomas following induction with known environmental carcinogens. Studies to date suggest the retinoblastoma gene/protein may play a role in oncogenesis in the medaka.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1996
- Accession Number
- ADA328820
Entities
People
- Brad Scoggins
- Gary K. Ostrander
- Jamess B. Blair
Organizations
- Oklahoma State University–Stillwater