Mapping of a Breast Carcinoma Tumor Suppressor Gene to Chromosome 11P15.5
Abstract
Loss of heterozygosity (LOH) on the short arm of chromosome 11 has been demonstrated in many cancers, suggesting the presence of a tumor suppressor gene. A number of studies have narrowed the region showing LOH in breast tumors to l1p15.5. The objectives of this study are: to screen breast tumor samples for LOH to further resolve the location of the putative tumor suppressor gene: and to screen candidate genes from this region. We are examining LOH in our region of interest using polymorphic markers. Samples from heterozygous individuals are examined from both normal and tumor tissue. We have isolated genomic DNA from 112 tissue samples, constituting 56 matched tumor and normal pairs. These samples are being analyzed for loss of heterozygosity (LOH) at three separate polymorphic markers, D11S1318, D11S4088, and D11S860. The analysis of marker D11S1318 is complete and is comparable to results which have been previously been reported at 6 percent LOH. To date D11S4088 is showing 12 percent LOH and D11S860 is incomplete.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA330191
Entities
People
- Tracey Moore
Organizations
- University of North Carolina at Chapel Hill