Characterization of Two Proteins which Interact with the BRCA1 Gene
Abstract
We have identified a novel protein, BAP1, which binds to the BRCAl RING finger domain (BRCA1-RF). BAP1 is a new, nuclear-localized, ubiquitin carboxyl-terminal hydrolase suggesting that deubiquitinating enzymes may play an important role in BRCA1 function. BAP1 binds to the wild-type BRCA1-RF both in vitro and in vivo, but not to mutant BRCA1-RF's found in tumors from breast cancer kindreds, or to related RING-finger proteins. BAP1 enhances BRCA1-mediated tumor suppression in colony formation assays and this activity requires the enzymatic function.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA330246
Entities
People
- Frank J. Rauscher
Organizations
- Wistar Institute