The Effect of Hyperthermia on Intracellular Sodium Concentration in Isolated Human Cells. A Preliminary Report.
Abstract
Groups of human squamous epithelial cells labeled with the sodium sensitive fluorescent dye, Sodium Green (Molecular Probes, OR) were subjected to a 20 minute hyperthermic stress at temperatures as high as 50 deg C and then cooled to 37 deg C. Changes in fluorescence were determined at one minute intervals using an interactive laser cytometer. Cells raised to temperatures in excess of 43 deg C showed a significant rise in fluorescence and thus a rise in intracellular sodium concentration, Na(+)i. Upon return to 37 deg C, Na(+)i in these cells did not fall but continued to rise at an increased rate compared to controls.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1996
- Accession Number
- ADA331123
Entities
People
- M. S. Koratich
- R. W. Hubbard
- S. L. Gaffin
Organizations
- United States Army Research Institute of Environmental Medicine