Regulation of Anchorage-Independent Growth in Breast Cancer: Role of Signalling by Extracellular Matrix and Growth Factors

Abstract

In this report we investigate the role of ECM proteins and growth factors in anchorage-independent survival and growth of a murine mammary carcinoma-derived cell line, designated SP1. We demonstrate that SP1 cells adhere well to FN and VN and express a number of integrins, including a5beta1, alpha v beta 3, alpha 6 beta 1, alpha 3 beta 1 and alpha 2 beta 1, the latter being upregulated under anchorage- independent conditions. We also demonstrated by partially removing FN from 7% FBS or adding FN to 1% FBS that FN is required for SP1 cell colony growth.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1997
Accession Number
ADA331130

Entities

People

  • Ron Saulnier

Organizations

  • Queen's University

Tags

DTIC Thesaurus Topics

  • Biomedical And Dental Materials
  • Breast Cancer
  • Carcinoma
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Health Services
  • Peptide Growth Factors
  • Polymeric Films
  • Tumor Cell Line

Readers

  • Molecular Biology and Genetics
  • Statistical inference.