Crystallation, X-Ray Structure Determination and Structure-Based Drug Design for Targeted Malarial Enzymes
Abstract
The aim of this project is to identify specific inhibitors of some enzymes of malaria parasite. Inhibitors will be designed based on the high-resolution crystal structure of individual target enzymes. We have already determined the crystal structure of P. falciparum lactate dehydrogenase, a key enzyme in the glycolytic pathway, to high resolution. This structure is currently being used for designing lead inhibitors. We have also purified PFPK-DHPS bifunctional enzyme for structure analysis and are presently screening for crystallization conditions. We have also optimized the purification of P. falciparum cyclophilin. We have also crystallized P. falciparum Rab6 for structure determination. This is the first Rab protein of the malaria parasite to be crystallized.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA331561
Entities
People
- Lawrence J. Delucas
Organizations
- University of Alabama