Glycosphingolipids as Putative Receptor for Staphylococcal Enterotoxin-B in Cultured Human Kidney Cells.

Abstract

Staphylococcal enterotoxin-B (SEB) is a common enterotoxin that can cause diarrhea and death in man. In these studies we have developed a specific and sensitive assay for the detection of SEB (enzyme linked receptor-based immunodot) in human fluids, plasma and urine. Our structure/function studies have revealed that amino acid sequence (130-160) of SEB (peptide #9) imparts toxic effects including cell death in PT cells. In addition, we found that SEB can activate neutral sphingomyelinase (N-SMase) resulting in the hydrolysis of sphingomyelin to ceramide and phosphocholine. Ceramide, in turn induces programmed cell death (apoptosis). Interestingly, several of the SEB mutants of peptide #9 were found to abrogate SEB toxicity in human kidney cells. Our findings will of potential value for the food industry, and to help determine toxemia in our soldiers. Such studies will also elaborate the pathophysiology of SEB induced toxemia in man.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1997
Accession Number
ADA332016

Entities

People

  • Subroto Chatterjee

Organizations

  • Johns Hopkins University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Apoptosis
  • Arteries
  • Biological Sciences
  • Biological Toxins
  • Blood
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Detection
  • Glycolipids
  • Health Services
  • Laboratory Animals
  • Liquid Chromatography
  • Lymphocytes
  • Medical Personnel
  • Programmed Cell Death

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and Cellular Biochemistry
  • Prostate Cancer Biology.