A Role for the NF-kb/Rel Transcription Factors in Human Breast Cancer

Abstract

Human breast cancer is characterized by the altered expression of growth factors, growth factor receptors and kinases. Downstream effectors of these molecules are transcription factors which presumably control aberrant gene expression regulating cell growth. We are studying the potential role of the transcription factor NF-kB in human breast cancer. Our rationale is based on the observations that NF-kB is activated in several human breast cancer cell lines and that certain repetitive DNA elements known as VNTRs, which bind NF-kB, are associated with breast cancer. Our data indicate that NF-kB is required for oncogenic transformation and that this transcription factor protects tumor cells against oncogene-induced and cancer therapy-induced apoptosis. Analysis of human breast cancer tumors indicate that NF-kB subunits p50, p52 and c-Rel are up-regulated in the great majority of tumors as compared to normal adjacent tissue. We are currently exploring whether these NF-kB forms bind to the VNTR elements and contribute to altered gene expression controlled by these elements. Functional experiments are underway to determine if altered NF-kB activity is involved in the initiation and progression of human breast cancer.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1997
Accession Number
ADA332161

Entities

People

  • Albert S Baldwin

Organizations

  • University of North Carolina at Chapel Hill

Tags

DTIC Thesaurus Topics

  • Apoptosis
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Techniques
  • Gene Expression
  • Genetics
  • Growth Factors
  • Medical Personnel
  • Neoplasms
  • Programmed Cell Death
  • Proteins
  • Stem Cells
  • Transcription Factors
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.