Improved Breast Cancer Detection Using a Novel In Situ Method to Visualize Clonality

Abstract

We report the successful development of a sensitive clonality assay with capabilities beyond the in situ fluorescent assay originally proposed. The assay is based on a novel method we have developed termed "biolithography" which applies photolithography, a process known in the semiconductor industry, to biologic material. Biolithogiaphy utilizes photoresist and targeted light irradiation to isolate DNA from selected cell populations under direct microscopic visualization with essentially 100% specIficity and subcellular resolution. Sensitivity is at the limit of PCR.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1997
Accession Number
ADA332325

Entities

People

  • John Zebala

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Androgen Receptors
  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cells
  • Chemistry
  • Chromosomes
  • Detection
  • Diseases And Disorders
  • Films
  • Genetics
  • Health Services
  • Materials
  • Medical Personnel
  • Molecules
  • Neoplasms

Readers

  • Molecular and genetic basis of cancer.
  • Nanofabrication and Microfabrication.
  • Nanoscale Plasmonic Nanotechnology

Technology Areas

  • Microelectronics
  • Microelectronics - Graphene