Evaluating Gene Therapy Approaches for the Prevention of Breast Cancer

Abstract

In order to determine the feasibility of using gene therapy for breast cancer prevention in the rat, lacZ recombinant adenoviral and retroviral vectors were infused into the mammary glands of rats in situ. Staining of whole glands with X-gal revealed the location of infected cells. Adenovirus infected the end buds with high efficiency but failed to infect the primary/secondary ductal cells. Expression of lacZ from the adenoviral vector was transient, with no expression by day 8 post-infusion.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1997
Accession Number
ADA332329

Entities

People

  • Michael N. Gould
  • Philip Watson

Organizations

  • University of Wisconsin–Madison

Tags

DTIC Thesaurus Topics

  • Biological Staining And Labeling
  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cell Line
  • Cells
  • Cellular Structures
  • Chemistry
  • Gene Therapy
  • Histological Techniques
  • Mammary Glands
  • Neoplasms
  • Peptides
  • Proteins
  • Therapy
  • Virotherapy
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Biology and Genetics
  • Molecular Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech