Genes Altered by Intracisternal A Particles in Mouse Mammary Tumorigenesis
Abstract
The purpose of the original proposal was to search for and isolate new transcripts associated with mammary carcinogenesis. In order to accomplish this we have been analyzing mammary carcinomas induced by 7,12-dimethylbenz (a) anthracene (DMBA) in BALB/c mice which express high levels of intracistemal A-particles (IAP). Differential hybpridization and differential display strategies are being used to isolate transcripts which contained IAP LTR sequences. A clone was isolated by library screens contained two poly adenylation signals and poly A tracts suggesting a rearrangement in library construction. Serendipitously, the unique portion of the clone hybridized to two transcripts upon Northern analysis, one tumor specifically. All tissues examined express the larger transcript while tumors of various etiologies express the smaller tumor associated transcript. Backcross analysis has localized the transcript to mouse chromosome 17.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA332372
Entities
People
- Michael R. Crowley
Organizations
- Health Research, Incorporated