Radiation-Induced Transformation in Human Breast Cells.
Abstract
Our study proposes to test the hypothesis that ionizing radiation can transform to a malignant phenotype immortalized/initiated breast cells from a donor with Li-Fraumeni syndrome (LFS) containing a germline mutation. Human breast cells were irradiated using a protocol similar to that used in the therapeutic treatment of breast carcinoma in situ. Exponentially growing cells were inoculated at 5 x 10 (5) per 75cm flask and irradiated 24 hrs later with 2Gy of gamma radiation at a dose rate of 2.37Gy/min. Additional doses of 2Gy were delivered at daily intervals to a total dose of 60Gy. After each lOGy increment, the cultures were allowed to recover for 7-10 days. After this recovery, a portion of the irradiated cultures were tested for changes in morphology, anchorage-independent growth, growth factor requirements (i.e. removal of BPE, EGF or insulin), growth in presence of semm and tumorigenicity. Potential transformants were tested for the development of the anchorage-independent phenotype following radiation treatment by suspension in 0.3% agar at a concentration of lxl04/ml and cells were examined for clonal growth at 21 days. In addition, three week old female SCID mice were insulated in the abdominal mammary fatpad with 10(7) unirradiated or radiation-treated human breast cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA332445
Entities
People
- Peter J. Thraves
Organizations
- Georgetown University