ErbB2 Regulation of c-src Activity in Breast Cancer
Abstract
In order to evaluate the effects of ErbB2 (neo) on c-src activation, nontumorigenic human mammary epithelial cells (184.A1 line) were neo- plastically transformed with ErbB2. Transformed cells exhibited 4-6 fold higher activity of c-src, measured as phosphorylation of a substrate peptide. However, there was no detectable change in c-src content of transformed cells. The activation of c-src was accompanied by increased phosphorylation of tyorsine 416, and by decreased phosphorylation of tyrosine 527, suggesting a kinase or phosphatase-mediated mechanism of c-src activation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA332502
Entities
People
- Lewisss G. Sheffield
Organizations
- University of Wisconsin–Madison