Role of a Placenta-Specific Gene in Mammary Tunorigenesis
Abstract
The mouse IAP-promoted placental gene (MIPP) is ectopically expressed in BALB/c mouse mammary carcinomas, making it a candidate oncogene. One purpose of the proposed research, therefore, is to show whether MIPP, as it appears in the mammary tumors, is oncogenic. Determining the function of MIPP and if the solo long terminal repeat (LTR) associated with the gene promotes its ectopic expression are also proposed. Finally, the proposed research includes studying the human homolog of MIPP (IPP) in breast cancers. Using RT-PCR, we have shown that MIPP transcripts in mammary tumors do not contain LTR sequences, and therefore are not promoted by the solo LTR. This experiment also showed that all MIPP transcripts contain the same 3' end. Degenerate oligonucleotide primed RT-PCR was used to amplify and clone part of the 5' region of MIPP from mammary tumors. A single open reading frame (orf), continuous with the orf at the 3' end, was found. This orf can code for an N terminal BTB/POZ protein/protein interaction domain and six C terminal kelch repeats. Therefore, MIPP may be an actin binding protein. RT-PCR analysis of normal and malignant human breast cell lines showed that IPP is not expressed in these cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1997
- Accession Number
- ADA332599
Entities
People
- Joshua N. Vanhouten
Organizations
- Health Research, Incorporated