Transgenic Mouse Model to Study the Role of EGF Receptor in Breast Cancer

Abstract

Epidermal growth factor (EGF) receptor is expressed in many normal tissues, including mammary epithelium. Overexpression or mutation of the EGF receptor cause neoplastic transformation in many cell types. Several studies have implicated the EGF receptor as a good prognostic marker in breast cancer, but its role in the etiology and progression of this malignancy is still under dispute. We propose to utilize a genetic approach to investigate this issue, by developing transgenic mice in which the EGF receptor gene in the mammary gland will be inactivated at the onset of first lactation. We have made the construct for the gene targeting and have 5 individual embryonic stem (ES) cells with potential correct DNA recombination to generate the loxP mouse. We also have made the final construct to generate white acid protein(WAP)-cre transgenic mouse.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1997
Accession Number
ADA334919

Entities

People

  • William Chen

Organizations

  • University of Illinois at Chicago

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cells
  • Chemistry
  • Dna Sequence Analysis
  • Genetic Structures
  • Glands
  • Growth Factors
  • Laboratory Animals
  • Mammary Glands
  • Materials
  • Mutations
  • Neoplasms
  • Proteins
  • Recombinant Dna
  • Targeting
  • Targets

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology