C-KIT AND Stem Cell Factor Expression in Breast Cancer
Abstract
We have proceeded to investigate some of the biologic consequences of c-kit and SCF coexpression since simple coexpression of receptor and ligand may imply, but does not prove, a potential autocrine(or paracrine) loop. We have therefore, constructed an autocrine loop by transfecting the breast cancer cell line, MCF7, that only expresses SCF, with a c-kit expression vector (21). MCF7 cells were transfected with both the c-kit expression vector and the empty pCEP4 vector (to serve as a control). Since the vector contained a hygromycin resistance gene, stable transfectants could be selected based on their survival in hygromycin-containing media.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1997
- Accession Number
- ADA335102
Entities
People
- Susan Hines
Organizations
- Virginia Commonwealth University