Molecular Mechanisms of Biosynthesis of Metal-Binding PCs
Abstract
Our research during the project period was directed towards three fundamental aspects of PCs: (1) metal-chelation by PCs, (2) sulfide complexes of PCs and (3) biosynthesis of PCs. We have made thorough investigations of the metal-binding properties of PCs and published data that provides benchmark information on the Ag(1), Cu(1), Cd(2), Hg(2) and Pb(2)-binding characteristics of these plant metal binding peptides. We have carried out detailed investigations on the sulfide complexes of PCs (PCs) that profoundly influence the metal-binding capacity of these peptides. In addition, these complexes have the potential to be very useful reagents for the detoxification of a variety of organic contaminants. Using a complementation cloning strategy, we have isolated a gene that is required for Cd tolerance and PC production. Although this gene did not turn out to be PC synthase, it provides us a good picture of the role of heme biosynthesis in PC production.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 31, 1997
- Accession Number
- ADA335807
Entities
People
- Rajesh Mehra
Organizations
- University of California, Riverside