The Interaction of Steroid Hormones and Oncogene in Breast Cancer
Abstract
We propose to generate a novel inducible bitransgenic system to examine the effect of the expression of a target oncogene, the polyoma middle T antigen, on the onset of mammary epithelium hyperplasia. Using this inducible system, the specific expression of a transgene can be initiated by the administration of an external compound. To establish such a regulatable system, we have introduced the regulator and the inducible target separately into mouse embryos to generate transgenic mouse lines. To target the expression of the regulator in mammary glands, we have placed the regulator under the control of the MMTV-LTR. Transgenic lines of the regulator and the target, polyoma middle T antigen have been generated. These lines are currently being crossed to generate bitransgenic lines and the expression of the target oncogene will be induced by administration of progesterone antagonists.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1997
- Accession Number
- ADA335989
Entities
People
- Sophia Y. Tsai
Organizations
- Baylor College of Medicine