Genomic Instability at Premalignant and Early Stages of Breast Cancer Development.
Abstract
We have developed techniques that allow the analysis of multiple chromosomal loci from single paraffin sections from breast cancer lesions. This approach is being used to allelotype small preinvasive breast cancer lesions. We identified the chromosome arms most frequently affected by allelic losses and imbalances at preinvasive stages of breast carcinogenesis and those allelic losses involved in more advanced stages of progression. We have now performed a high resolution allelotype of chromosome 16q. This allowed us to refine the location of specific subchromosome regions containing putative tumor suppressor genes of relevance on early breast carcinogenesis. We also performed a chromosome 16 high resolution allelotype of a panel of human breast cancer lines in order to identify areas of hemizygosity and homozygous loss. As a natural and important extension of these studies we have built a contig of yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) clones spanning the chromosome 16q region in which frequent allelic losses were detected. In studies in progress we have isolated numerous cDNA clones from a human breast epithelial library that map to the region of interest.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1997
- Accession Number
- ADA336689
Entities
People
- C M Aldaz
Organizations
- University of Texas at Austin