Oligosaccharide Markers for Prognosis of Low-Risk Breast Cancer.
Abstract
Progress to date has included the determination of the occurrence of several different cell surface oligosaccharides and proteases on sections from 181 different ductal breast carcinomas. Monoclonal antibodies specific for the blood group related oligosaccharides Lea, LeX, sialyl- Lea, LeY, Tn, Sialyl Tn and extended Lea - LeX were used to define the cell surface oligosaccharides and both immunofluorescence techniques and immuno streptavadin-biotin techniques were used to evaluate the fraction of tumor cells that are positive and the relative amount of reaction product as measured using an image processing system. The preliminary analysis of the available data has shown that the extended Lea-LeX oligosaccharide is a statistically significant indicator of poor prognosis following surgical removal of small, node-negative ductal breast carcinomas. cDNA libraries constructed from cells that express Lea-LeX have been screened and selected for cDNAs that alter the expression of this oligosaccharide in cancer cells. Three candidate genes have been identified and preliminarily characterized.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1997
- Accession Number
- ADA337015
Entities
People
- David E. Pettijohn
Organizations
- University of Colorado Health