Guidance for Performance of the H4IIE Dioxin Screening Assay.

Abstract

This technical note provides protocols for maintenance of cell cultures and for the conduct of a biomarker-based screening assay for dioxin toxic equivalents (TCDD TEQs) in sediments and other environmental samples using the H4IIE rat hepatoma cell line as performed at the U.S. Army Engineer Waterways Experiment Station (WES). The purpose of these protocols is to provide analysts who may wish to gain the capability for performing the assay with a detailed 'blueprint' of how the assay is conducted and of what is necessary in terms of technique, materials, instrumentation, and time.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1998
Accession Number
ADA338374

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DTIC Thesaurus Topics

  • Alcohols
  • Amino Acids
  • Aromatic Hydrocarbons
  • Birds
  • Cell Line
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Environmental Protection
  • Fish
  • Great Lakes
  • Hydrocarbons
  • Medical Personnel
  • Organic Chemistry
  • Polychlorinated Biphenyls
  • Wildlife

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