A Quantitative Multimarker Molecular Assay to Detect Hemtogenous Breast Cancer Metastasis

Abstract

Clinical detection of tumor progression is a major problem in treatment decisions for breast cancer patients. The ability to accurately detect hematogenous metastasis should significantly aid oncologist in disease management. To identify tumor progression more effectively than current approaches a less invasive, more accurate, quantitative assay is needed. Detection of metastatic tumor cells in blood and tissue by single marker Reverse- Transcriptase Polymerase Chain Reaction (RT-PCR) has shown potential value in clinical diagnosis. We have successfully developed unique approach using multimarker, quantitative analysis and automated Southern blot detection system to provide highly stringent large scale analysis. Our experience in other cancers and viral nucleic detection will be applied to breast cancer. In this proposal we plan to test our hypothesis that a multimarker molecular assay can be clinically useful in detection hematogenous metastasis in breast cancer patients of different clinical and pathological stages of disease. The objective is to improve diagnosis and monitor patients for metastatic disease.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1997
Accession Number
ADA338716

Entities

People

  • Dave S. Hoon

Tags

DTIC Thesaurus Topics

  • Blood
  • Breast Cancer
  • Carcinoma
  • Cells
  • Chain Reactions
  • Chemical Reactions
  • Detection
  • Diseases And Disorders
  • Epithelial Cells
  • Gene Expression
  • Genetics
  • Medical Personnel
  • Mrna
  • Neoplasms
  • Oncology
  • Physicians
  • Polymerase Chain Reaction

Fields of Study

  • Biology
  • Medicine

Readers

  • Oncology (Cancer Research).
  • Oncology and Biomarker-Based Cancer Detection.