Actions and Substrates for the HER4 Tyrosine Kinase in Breast Cancer
Abstract
During the first year of our HER4 and breast cancer grant, we have made progress in methods, cell line and reagent development. We have made MDA-MB 453 and 32D cell lines that stably express the Epidermal Growth Factor (EGF) receptor:Human EGF receptor 4 (HER4) chimera. Antisera directed against the chimera have demonstrated its expression. These 2 transfected cell lines will be used to compare HER4 and EGF receptor tyrosine phosphorylated substrates. Activation of the EGF receptor:HER4 chimera by adding EGF to transfected 32D cells stops their growth. This result suggest that our overarching hypothesis, i.e., the HER4 signal is different from that of the EGF receptor, is correct. To isolate downstream HER4 signaling elements, we are creating recombinant molecules as bait for use in the yeast 2 hybrid system. We are testing the ability of these constructs to be tyrosine phosphorylated when expressed in yeast. Lastly, generation of a HER4 antibody should allow us to do Western blot analysis of HER4 content in a variety of breast cancer samples, allowing attempts to correlate HER4 expression and breast cancer prognosis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADA338722
Entities
People
- H. S. Earp
Organizations
- University of North Carolina at Chapel Hill