Role of Seprase in Breast Cancer
Abstract
This research investigates the role of the serine integral membrane protease seprase in human breast cancer cell invasion. The project goals are to identify and characterize a full-length cDNA fur human breast cancer seprase and to investigate the contribution of seprase to the metastatic potential of breast cells. Progress made in the first year of this four year project is reported. Chicken embryo cDNA libraries have been prepared and screened with the polyclonal antibody against chicken embryo seprase that intensely labels human breast cancer cells. This probe identified a novel gene encoding a protein of unknown function. A reverse transcriptase- polymerase chain reaction (RT-PCR) strategy for cloning the seprase gene was developed based on conserved DNA sequences of human nonclassical serine proteases. A 250 bp cDNA with 85 % homology to catalytic domain of the family of serine integral membrane proteases that includes seprase was produced by RT-PCR of chicken embryo RNA.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1997
- Accession Number
- ADA338776
Entities
People
- Thomas J. Kelly Jr.
Organizations
- University of Arkansas at Little Rock