Inhibition of Breast Cancer Growth by IGFBP-3

Abstract

The proposal of my grant is to investigate the biological significance and mechanism of IGFBP-3 as well as identification and characterization of the IGFBP-3 receptor in human breast cancer cells. As a first year task, I initiated the identification of the putative IGFBP-3 receptor in human breast cancer cells by employing the yeast two-hybrid system. To date this approach has been successful using total Hs578T breast cancer cell mRNA for generation of a cDNA library, and a cDNA encoding an IGFBP-3 fragment IGFBP-3 sup 88-148 as a "bait". Preliminary screening of transformant colonies was successful, yielding several hundred positive interaction clones.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1997
Accession Number
ADA338865

Entities

People

  • Youngman Oh

Organizations

  • Oregon Health & Science University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical And Dental Materials
  • Blood
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Media
  • Genetic Structures
  • Growth Factors
  • Immune Serums
  • Molecular Biology
  • Neoplasms
  • Polymer Chemistry
  • Polymeric Films
  • Sequence Analysis

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Oncology (Cancer Research).
  • Optical Fiber Sensing and Electromagnetic Propagation.