Genetic Determinants of Sindbis Virus Oral Infectivity in Aedes Aegypti Mosquitoes

Abstract

The TE/3'2J double subgenomic Sindbis viruses (dsSIN) have been used to express genes in Aedes Aegypti nerve and salivary gland tissues. However, TE/3'2J viruses must be intrathoracically inoculated into the mosquitoes and exogenous expression in the mosquito midgut has not been possible. To improve oral infectivity, serial oral passage with selection for variants that disseminated to neural tissues resulted in an eight-fold increase in dissemination rate and enhanced tropism for mosquito midgut epithelium. Sequencing of the glycoprotein- encoding genes from the passaged virus revealed three consensus amino acid changes.

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Document Details

Document Type
Technical Report
Publication Date
Mar 09, 1998
Accession Number
ADA339032

Entities

People

  • Robert C. Seabaugh

Organizations

  • Air Force Institute of Technology

Tags

DTIC Thesaurus Topics

  • Animal Structures
  • Arbovirus Infections
  • Cells
  • Chemistry
  • Dna Sequence Analysis
  • Equine Encephalitis
  • Genetic Engineering
  • Genetic Variation
  • Genetics
  • Health Services
  • Proteins
  • Viral Structures
  • Virus Diseases
  • Viruses

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology
  • Molecular Genetics
  • Vector-Borne Disease and Entomology

Technology Areas

  • Biotechnology